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位置: 首页 > 磷酸化抗体 > C/EBP-α (Phospho-Ser21) Antibody

C/EBP-α (Phospho-Ser21) Antibody#11648

C/EBP-α (Phospho-Ser21) Antibody
C/EBP-α (Phospho-Ser21) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称C/EBP-α (Phospho-Ser21) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of C/EBP-α only when phosphorylated at serine 21.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C/EBP-α.

基因/蛋白名称C/EBP-α

修饰Phospho

别名CAP4; ICE8; MACH; MCH5; Mch-5

数据库入口号Swiss-Prot#: P49715;
NCBI Gene#: 1050;
NCBI Protein#: NP_004355.2.

UniprotP49715

实际分子量45kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HepG2 cells treated with EGF using C/EBP-α (phospho-Ser21) Antibody #11648.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using C/EBP-α (phospho-Ser21) antibody #11648 (left)or the same antibody preincubated with blocking peptide (right).
背景

C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Takashi Akasaka, Blood, Apr 2007; 109: 3451 - 3461.
Carolina Gillio-Meina, Biol Reprod, May 2005; 72: 1194 - 1204.
Rapha?l M¨¦tivier, Mol. Endocrinol., Nov 2001; 15: 1953.
Neoncheol Jung, Eur. J. Biochem., Oct 2000; 267: 6180.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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