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位置: 首页 > 磷酸化抗体 > Caspase 9 (Phospho-Thr125) Antibody

Caspase 9 (Phospho-Thr125) Antibody#11649

Caspase 9 (Phospho-Thr125) Antibody
Caspase 9 (Phospho-Thr125) Antibody
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产品总价:
产品详情

产品名称Caspase 9 (Phospho-Thr125) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R).

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9.

基因/蛋白名称Caspase 9

修饰Phospho

别名 APAF-3; MCH6; RNCASP9; CASP-9; ICE-LAP6

数据库入口号Swiss-Prot#: P55211;
NCBI Gene#: 842;
NCBI Protein#: NP_001220.2.

UniprotP55211

实际分子量47kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody #11649.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody #11649 (left)or the same antibody preincubated with blocking peptide (right).
背景

Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

Duan H., J. Biol. Chem. 271:16720-16724(1996).
Srinivasula S.M., J. Biol. Chem. 271:27099-27106(1996).
Hadano S., Mamm. Genome 10:757-760(1999)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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