产品详情
产品名称GSK3β(Phospho-Ser9) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of GSK3 beta only when phosphorylated at serine 9.
免疫原类型Peptide-KLH
免疫原描述Synthesized phospho-peptide around the phosphorylation site of human GSK3β (phospho Ser9)
基因/蛋白名称GSK3β
修饰Phospho
别名Factor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta
数据库入口号Swiss-Prot: P49841
NCBI Protein: NP_x.1
UniprotP49841
实际分子量46kd
浓度1.0mg/ml
配方Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
保存Store at -20°C for long term preservation (recommended). Avoid Store at 4°C for short term use.
应用详情
Predicted MW: 46kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3β(Phospho-Ser9) Antibody #11002.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002 (left) or the same antibody preincubated with blocking peptide #51002 (right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β(Phospho-Ser9) Antibody #11002.
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002.
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β (Phospho-Ser9) Antibody #11002.
Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3β (Phospho-Ser9) Antibody #11002.
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