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位置: 首页 > 磷酸化抗体 > p90 RSK (Phospho-Thr573) Antibody

p90 RSK (Phospho-Thr573) Antibody#11665

p90 RSK (Phospho-Thr573) Antibody
p90 RSK (Phospho-Thr573) Antibody
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产品总价:
产品详情

产品名称p90 RSK (Phospho-Thr573) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of P90RSK only when phosphorylated at threonine 573.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 573(L-M-T(p)-P-C) derived from Human p90 RSK.

基因/蛋白名称p90 RSK

修饰Phospho

别名KS6A1; p90RSK1; RSK1; RPS6KA1;

数据库入口号Swiss-Prot#: Q15418;
NCBI Gene#: 6195;
NCBI Protein#: NP_002944.2.

UniprotQ15418

实际分子量95kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 293 cells treated with UV using p90 RSK (Phospho-Thr573) Antibody #11665.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody #11665 (left)or the same antibody preincubated with blocking peptide (right).
背景

Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes.

Eiichi Takahashi, J. Biol. Chem., Jul 1999; 274: 20206.
Ye Zhang, J. Biol. Chem., May 2002; 277: 19042 - 19048.
Ganesh R. Panta, Mol. Cell. Biol., Mar 2004; 24: 1823 - 1835.

如果您使用该产品11665发表了文章,请通知我们,让我们可以引用您的文献。

et al,Response of MAPK pathway to iron oxide nanoparticles in vitro treatment promotes osteogenic differentiation of hBMSCs.In Biomaterials.On 2016 Apr by Wang Q, Chen B et al..PMID:26874888, , (2016),
PMID: 26874888
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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