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位置: 首页 > 磷酸化抗体 > PEA-15 (Phospho-Ser104) Antibody

PEA-15 (Phospho-Ser104) Antibody#11676

PEA-15 (Phospho-Ser104) Antibody
PEA-15 (Phospho-Ser104) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PEA-15 (Phospho-Ser104) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 104(I-P-S(p)-A-K) derived from Human PEA-15.

基因/蛋白名称PEA-15

修饰Phospho

别名PE15; PEA15; PED;

数据库入口号Swiss-Prot#: Q15121;
NCBI Gene#: 8682;
NCBI Protein#: NP_003759.1.

UniprotQ15121

实际分子量19kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from COS cells treated with TNF using PEA-15 (Phospho-Ser104) Antibody #11676.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human heart tissue using PEA-15 (Phospho-Ser104) antibody #11676 (left)or the same antibody preincubated with blocking peptide (right).
背景

Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm. Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.

Estelles A., J. Biol. Chem. 271:14800-14806(1996).
Condorelli G., EMBO J. 17:3858-3866(1998).
Wolford J.K., Gene 241:143-148(2000).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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