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位置: 首页 > 磷酸化抗体 > Ras-GRF1 (Phospho-Ser916) Antibody

Ras-GRF1 (Phospho-Ser916) Antibody#11678

Ras-GRF1 (Phospho-Ser916) Antibody
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产品总价:
产品详情

产品名称Ras-GRF1 (Phospho-Ser916) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 916(R-M-S(p)-L-A) derived from Mouse Ras-GRF1.

基因/蛋白名称Ras-GRF1

修饰Phospho

别名CDC25; GNRP; GRF1; RASGRF1;

数据库入口号Swiss-Prot#: P27671;
NCBI Gene#: 19417;
NCBI Protein#: NP_002882.3.

UniprotP27671

实际分子量145kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using Ras-GRF1 (Phospho-Ser916) antibody #11678 (left)or the same antibody preincubated with blocking peptide (right).
背景

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.

Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285.
Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189.
Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379.
Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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