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位置: 首页 > 磷酸化抗体 > Bloom Syndrome (Phospho-Thr99) Antibody

Bloom Syndrome (Phospho-Thr99) Antibody#11681

Bloom Syndrome (Phospho-Thr99) Antibody
Bloom Syndrome (Phospho-Thr99) Antibody
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产品总价:
产品详情

产品名称Bloom Syndrome (Phospho-Thr99) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of Bloom Syndrome Protein only when phosphorylated at threonine 99.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 99 (Q-E-T(p)-Q-R) derived from Human Bloom Syndrome.

基因/蛋白名称Bloom Syndrome

修饰Phospho

别名 RECQ2; RECQL3; type 2; EC 3.6.1; RecQ protein-like 3

数据库入口号Swiss-Prot#: P54132;
NCBI Gene#: 641;
NCBI Protein#: NP_000048.1.

UniprotP54132

实际分子量159kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HepG2 cells using Bloom Syndrome Protein (Phospho-Thr99) Antibody #11681.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human heart tissue, using Bloom Syndrome Protein (Phospho-Thr99) antibody #11681 (left)or the same antibody preincubated with blocking peptide (right).
背景

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE).

Ellis N.A., Cell 83:655-666(1995).
Karow J.K., J. Biol. Chem. 272:30611-30614(1997).
Pichierri P., EMBO J. 23:3154-3163(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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