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位置: 首页 > 磷酸化抗体 > B-RAF (Phospho-Thr599) Antibody

B-RAF (Phospho-Thr599) Antibody#11682

B-RAF (Phospho-Thr599) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称B-RAF (Phospho-Thr599) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu

特异性The antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 599 (L-A-T(p)-V-K) derived from Human B-RAF.

基因/蛋白名称B-RAF

修饰Phospho

别名B-RAF; RAFB1; RMIL; BRAF1; c-RMIL

数据库入口号Swiss-Prot#: P15056;
NCBI Gene#: 673;
NCBI Protein#: NP_004324.2.

UniprotP15056

实际分子量84kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using B-Raf (Phospho-Thr598) antibody #11682 (left)or the same antibody preincubated with blocking peptide (right).
背景

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Armelle Calipel, J. Biol. Chem., Oct 2003; 278: 42409 - 42418.
Tara J. Dillon, J. Biol. Chem., Sep 2003; 278: 35940 - 35949.
R K Jaiswal, Mol. Cell. Biol., Oct 1994; 14: 6944 - 6953.
Laura L. Dugan, J. Biol. Chem., Sep 1999; 274: 25842.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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