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位置: 首页 > 磷酸化抗体 > BAD (Phospho-Ser91/128) Antibody

BAD (Phospho-Ser91/128) Antibody#11685

BAD (Phospho-Ser91/128) Antibody
BAD (Phospho-Ser91/128) Antibody
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产品总价:
产品详情

产品名称BAD (Phospho-Ser91/128) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of BAD only when phosphorylated at serine 91/ serine 128.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 128 (E-L-S(p)-P-F) derived from Mouse BAD.

基因/蛋白名称BAD

修饰Phospho

别名 BAD; BBC6; BCL2L8; Bcl- XL/Bcl-2 associated death promoter;

数据库入口号Swiss-Prot#: Q92934;
NCBI Gene#: 572;
NCBI Protein#: NP_004313.1.

UniprotQ92934

实际分子量22kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from COS7 cells treated with TNF-a using BAD (Phospho-Ser91/128) Antibody #11685.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Phospho-Ser91/128) antibody #11685 (left)or the same antibody preincubated with blocking peptide (right).
背景

The protein encoded by BAD gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Wang H.-G., Cell 87:629-638(1996).
Ottilie S., J. Biol. Chem. 272:30866-30872(1997).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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