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位置: 首页 > 磷酸化抗体 > Cyclin H (Phospho-Thr315) Antibody

Cyclin H (Phospho-Thr315) Antibody#11689

Cyclin H (Phospho-Thr315) Antibody
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产品总价:
产品详情

产品名称Cyclin H (Phospho-Thr315) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous levels of Cyclin H only when phosphorylated at threonine 315.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 315 (E-W-T(p)-D-D) derived from Human Cyclin H .

基因/蛋白名称Cyclin H

修饰Phospho

别名cyclin H; p34; p37; MO15-associated protein;

数据库入口号Swiss-Prot#: P51946;
NCBI Gene#: 902;
NCBI Protein#: NP_001230.1.

UniprotP51946

实际分子量36kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from JK cells (Lane 2), using Cyclin H (Phospho-Thr315) Antibody #11689. The lane on the left is treated with antigen-specific peptide.
背景

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Maekelae T.P., Nature 371:254-257(1994).
Fisher R.P., Cell 78:713-724(1994).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

如果您使用该产品11689发表了文章,请通知我们,让我们可以引用您的文献。

et al,CyclIns B1, T1, and H differ In their molecular mode of Interaction with cytomegalovirus proteIn kInase pUL97. In J Biol Chem on 2019 Apr 12 by SteIngruber M, Keller L, et al.. PMID:30782840, , (2019),
PMID: 30782840
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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