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位置: 首页 > 磷酸化抗体 > Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody

Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody#11704

Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody
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产品总价:
产品详情

产品名称Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of Nuclear Receptor NR4A1 only when phosphorylated at serine 351

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine351(L-P-S(p)-K-P) derived from Human Nuclear Receptor NR4A1.

基因/蛋白名称Nuclear Receptor NR4A1

修饰Phospho

别名NGFIB; GFRP1; HMR; NR4A1; NAK1

数据库入口号Swiss-Prot#: P22736;
NCBI Gene#: 3164;
NCBI Protein#: NP_002126.2.

UniprotP22736

实际分子量64kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody #11704 (left)or the same antibody preincubated with blocking peptide (right).
背景

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.

Nakai A., Mol. Endocrinol. 4:1438-1443(1990).
Chang C., J. Steroid Biochem. 34:391-395(1989).
Bondy G.P., Cell Growth Differ. 2:203-208(1991)

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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