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位置: 首页 > 磷酸化抗体 > p53 (Phospho-Thr387) Antibody

p53 (Phospho-Thr387) Antibody#11718

p53 (Phospho-Thr387) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称p53 (Phospho-Thr387) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC;IF;ELISA

种属反应性Hu Rt Ms

特异性The antibody detects endogenous levels of p53 only when phosphorylated at threonine 387.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 387(F-K-T(p)-E-G) derived from Human p53.

基因/蛋白名称p53

修饰Phospho

别名TP53; tumor protein p53; Tumor suppressor p53;

数据库入口号Swiss-Prot#: P04637;
NCBI Gene#: 7157;
NCBI Protein#: NP_000537.3.

UniprotP04637

实际分子量43kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Thr387) antibody #11718 (left)or the same antibody preincubated with blocking peptide (right).
背景

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Zakut-Houri R., EMBO J. 4:1251-1255(1985).
Lamb P., Mol. Cell. Biol. 6:1379-1385(1986).
Harlow E., Mol. Cell. Biol. 5:1601-1610(1985).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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