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位置: 首页 > 磷酸化抗体 > LATS1/2 (Phospho-Thr1079/1041) Antibody

LATS1/2 (Phospho-Thr1079/1041) Antibody#11736

LATS1/2 (Phospho-Thr1079/1041) Antibody
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产品总价:
产品详情

产品名称LATS1/2 (Phospho-Thr1079/1041) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of LATS1/2 only when phosphorylated at threonine 1079/1041.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 1079/1041 (E-F-T(p)-F-R) derived from Human LATS1/2 .

基因/蛋白名称LATS1/2

修饰Phospho

别名WARTS; Large tumor suppressor 1; EC 2.7.11.1;

数据库入口号Swiss-Prot#: O95835/Q9NRM7;
NCBI Gene#: 9113/26524;
NCBI Protein#: NP_004681.1.

UniprotO95835/Q9NRM7

实际分子量126kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using LATS1/2 (Phospho-Thr1079/1041) antibody #11736 (left)or the same antibody preincubated with blocking peptide (right).
背景

The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding.

Tao W., Nat. Genet. 21:177-181(1999).
Nishiyama Y., FEBS Lett. 459:159-165(1999).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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