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位置: 首页 > 磷酸化抗体 > MER/SKY (Phospho-Tyr749/681) Antibody

MER/SKY (Phospho-Tyr749/681) Antibody#11740

MER/SKY (Phospho-Tyr749/681) Antibody
MER/SKY (Phospho-Tyr749/681) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称MER/SKY (Phospho-Tyr749/681) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Human,Mouse,Rat, Chicken, Zebrafish

特异性The antibody detects endogenous levels of MER/SKY only when phosphorylated at tyrosine 749/681.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 749/681(K-I-Y(p)-S-G) derived from Human MER/SKY .

基因/蛋白名称MER/SKY

修饰Phospho

别名C-mer; MERK; MERTK;

数据库入口号Swiss-Prot#: Q12866/Q06418;
NCBI Gene#: 10461/7301;
NCBI Protein#: NP_006334.2.

UniprotQ12866/Q06418

实际分子量97kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 3T3 cells treated with EGF using MER/SKY (Phospho-Tyr749/681) Antibody #11740.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using MER/SKY (Phospho-Tyr749/681) antibody #11740 (left)or the same antibody preincubated with blocking peptide (right).
背景

This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP).

Graham D.K., Cell Growth Differ. 5:647-657(1994).
Gal A., Nat. Genet. 26:270-271(2000).
Hillier L.W., Nature 434:724-731(2005).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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