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位置: 首页 > 磷酸化抗体 > TOP2A (Phospho-Thr1343) Antibody

TOP2A (Phospho-Thr1343) Antibody#11763

TOP2A (Phospho-Thr1343) Antibody
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产品总价:
产品详情

产品名称TOP2A (Phospho-Thr1343) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of TOP2A only when phosphorylated at threonine 1343.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine1343(E-K-T(p)-D-D) derived from Human TOP2A.

基因/蛋白名称TOP2A

修饰Phospho

别名TP2A; TOP2; TOP2A; P11388-1;

数据库入口号Swiss-Prot#: P11388;
NCBI Gene#: 7153;
NCBI Protein#: NP_001058.2.

UniprotP11388

实际分子量190kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 cells treated with Ca2+ using TOP2A (Phospho-Thr1343) Antibody #11763.The lane on the right is treated with the antigen-specific peptide.
背景

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event.

Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988).
Wasserman R.A., Cancer Res. 53:3591-3596(1993).
Lang A.J., Gene 221:255-266(1998).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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