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位置: 首页 > 磷酸化抗体 > FOS (Phospho-Thr232) Antibody

FOS (Phospho-Thr232) Antibody#11764

FOS (Phospho-Thr232) Antibody
FOS (Phospho-Thr232) Antibody
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产品总价:
产品详情

产品名称FOS (Phospho-Thr232) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of FOS only when phosphorylated at threonine 232.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 232(V-A-T(p)-P-E) derived from Human FOS .

基因/蛋白名称FOS

修饰Phospho

别名FOS; G0S7; Cellular oncogene fos;

数据库入口号Swiss-Prot#: P01100;
NCBI Gene#: 2353;
NCBI Protein#: NP_005243.1.

UniprotP01100

实际分子量48kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from COS7 cells (Lane 2), using FOS (Phospho-Thr232) Antibody #11764. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOS (Phospho-Thr232) antibody #11764 (left)or the same antibody preincubated with blocking peptide (right).
背景

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

van Straaten F. Proc. Natl. Acad. Sci. U.S.A. 80:3183-3187(1983).
Hai T., Genes Dev. 3:2083-2090(1989).
Heilig R., Nature 421:601-607(2003).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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