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位置: 首页 > 磷酸化抗体 > BL-CAM (Phospho-Tyr807) Antibody

BL-CAM (Phospho-Tyr807) Antibody#11765

BL-CAM (Phospho-Tyr807) Antibody
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产品总价:
产品详情

产品名称BL-CAM (Phospho-Tyr807) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of BL-CAM only when phosphorylated at tyrosine 807.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 807 (G-D-Y(p)-E-N) derived from Human BL-CAM.

基因/蛋白名称BL-CAM

修饰Phospho

别名Siglec-2; B-cell receptor CD22; BL-CAM; Leu-14; B-lymphocyte cell adhesion molecule

数据库入口号Swiss-Prot#: P20273;
NCBI Gene#: 933;
NCBI Protein#: NP_001762.2.

UniprotP20273

实际分子量95kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from COS7 cells (Lane 2), using BL-CAM (Phospho-Tyr807) Antibody #11765.The lane on the left is treated with antigen-specific peptide.
背景

Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Wilson G.L., J. Exp. Med. 173-137-146(1991).
Wilson G.L., J. Immunol. 150:5013-5024(1993).
Stamenkovic I., Nature 345-74-77(1990).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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