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位置: 首页 > 磷酸化抗体 > FGFR1/2 (Phospho-Tyr463/466) Antibody

FGFR1/2 (Phospho-Tyr463/466) Antibody#11772

FGFR1/2 (Phospho-Tyr463/466) Antibody
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产品总价:
产品详情

产品名称FGFR1/2 (Phospho-Tyr463/466) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous levels of FGFR1/2 only when phosphorylated at tyrosine 463/466.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 463/466(S-E-Y(p)-E-L) derived from Human FGFR1/2.

基因/蛋白名称FGFR1/2

修饰Phospho

别名BFGFR; CEK; FLG; CD331; FLT2

数据库入口号Swiss-Prot#: P11362/P21802;
NCBI Gene#: 2260/2263;
NCBI Protein#: NP_075598.2.

UniprotP11362/P21802

实际分子量91kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 cells using FGFR1/2 (Phospho-Tyr463/466) Antibody #11772.The lane on the right is treated with the antigen-specific peptide.
背景

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation.

Itoh N., Biochem. Biophys. Res. Commun. 169:680-685(1990).
Dionne C.A., EMBO J. 9:2685-2692(1990).
Johnson D.E., Mol. Cell. Biol. 10:4728-4736(1990).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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