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位置: 首页 > 磷酸化抗体 > WWOX (Phospho-Tyr33) Antibody

WWOX (Phospho-Tyr33) Antibody#11779

WWOX (Phospho-Tyr33) Antibody
WWOX (Phospho-Tyr33) Antibody
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产品总价:
产品详情

产品名称WWOX (Phospho-Tyr33) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of WWOX only when phosphorylated at tyrosine 33.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 33 (W-V-Y(p)-Y-A) derived from Human WWOX.

基因/蛋白名称WWOX

修饰Phospho

别名FOR; WOX1; Aberrant WW domain-containing oxidoreductase;

数据库入口号Swiss-Prot#: Q96KM3;
NCBI Gene#: 51741;
NCBI Protein#: NP_057457.1.

UniprotQ96KM3

实际分子量55kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HepG2 cells treated with PMA using WWOX (Phospho-Tyr33) Antibody #11779.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using WWOX (Phospho-Tyr33) antibody #11779 (left)or the same antibody preincubated with blocking peptide (right).
背景

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 2 WW domains and a short-chain dehydrogenase/reductase domain (SRD). The highest normal expression of this gene is detected in hormonally regulated tissues such as testis, ovary, and prostate. This expression pattern and the presence of an SRD domain suggest a role for this gene in steroid metabolism.

Bednarek A.K., Cancer Res. 60:2140-2145(2000).
Ried K., Hum. Mol. Genet. 9:1651-1663(2000).
Bednarek A.K., Cancer Res. 61:8068-8073(2001).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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