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位置: 首页 > 磷酸化抗体 > Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody

Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody#11780

Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody
Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of Ephrin B1/B2/B3 only when phosphorylated at tyrosine 324.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 324 (G-D-Y(p)-G-H) derived from Human Ephrin B1/B2/B3 .

基因/蛋白名称Ephrin B1/B2/B3

修饰Phospho

别名EPL2; CEL5-L; EFL-3; EFNB1; ELK-L

数据库入口号Swiss-Prot#: P98172/P52799/Q15768;
NCBI Gene#: 1947/1948/1949;
NCBI Protein#: NP_004420.1.

UniprotP98172/P52799/Q15768

实际分子量46kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from K562 cells treated with serum using Ephrin B1/B2/B3 (Phospho-Tyr324) Antibody #11780.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Ephrin B1/B2/B3 (Phospho-Tyr324) antibody #11780 (left)or the same antibody preincubated with blocking peptide (right).
背景

This gene encodes a member of the ephrin family. The encoded protein is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system.

Beckmann M.P., EMBO J. 13:3757-3762(1994).
Davis S., Science 266:816-819(1994).
Fletcher F.A., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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