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位置: 首页 > 磷酸化抗体 > ATF-2 (Phospho-Ser472) Antibody

ATF-2 (Phospho-Ser472) Antibody#11785

ATF-2 (Phospho-Ser472) Antibody
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产品总价:
产品详情

产品名称ATF-2 (Phospho-Ser472) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu

特异性The antibody detects endogenous levels of ATF-2 only when phosphorylated at serine 472.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 472 (A-L-S(p)-Q-I) derived from Human ATF-2.

基因/蛋白名称ATF-2

修饰Phospho

别名Activating 2; ATF2; cAMP response element binding protein CRE- BP1; CREB2; HB16

数据库入口号Swiss-Prot#: P15336;
NCBI Gene#: 1386;
NCBI Protein#: NP_001243019.1.

UniprotP15336

实际分子量54kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using ATF-2 (Phospho-Ser472) antibody #11785 (left)or the same antibody preincubated with blocking peptide (right).
背景

Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.

Maekawa T., EMBO J. 8:2023-2028(1989).
Yang L., J. Immunol. 158:2522-2525(1997).
Kara C.J., Mol. Cell. Biol. 10:1347-1357(1990).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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