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位置: 首页 > 磷酸化抗体 > BRCA1 (Phospho-Ser1457) Antibody

BRCA1 (Phospho-Ser1457) Antibody#11787

BRCA1 (Phospho-Ser1457) Antibody
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产品总价:
产品详情

产品名称BRCA1 (Phospho-Ser1457) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of BRCA1 only when phosphorylated at serine 1457.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 1457 (L-T-S(p)-Q-K) derived from Human BRCA1.

基因/蛋白名称BRCA1

修饰Phospho

别名BRCA1; Breast cancer type 1 susceptibility protein; familial breast/ovarian cancer gene 1;

数据库入口号Swiss-Prot#: P38398;
NCBI Gene#: 672;
NCBI Protein#: NP_009225.1.

UniprotP38398

实际分子量220kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from 293 cells (Lane 2), using BRCA1 (Phospho-Ser1457) Antibody #11787. The lane on the left is treated with antigen-specific peptide.
背景

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

Miki Y., Science 266:66-71(1994).
Smith T.M., Genome Res. 6:1029-1049(1996).
Wilson C.A., Oncogene 14:1-16(1997).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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