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位置: 首页 > 磷酸化抗体 > C/EBP-α (Phospho-Thr230) Antibody

C/EBP-α (Phospho-Thr230) Antibody#11788

C/EBP-α (Phospho-Thr230) Antibody
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产品总价:
产品详情

产品名称C/EBP-α (Phospho-Thr230) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The Antibody detects endogenous levels of C/EBP-α only when phosphorylated at Thr230.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 230 (P-P-T(p)-P-V) derived from Human C/EBP-α.

基因/蛋白名称C/EBP-α

修饰Phospho

别名CCAAT/enhancer-binding protein alpha C/EBP alpha CEBPA;

数据库入口号Swiss-Prot#: P49715;
NCBI Gene#: 1050;
NCBI Protein#: NP_004355.2.

UniprotP49715

实际分子量42kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from cos-7 cells (Lane 2), using C/EBP-α (Phospho-Thr230) Antibody #11788. The lane on the left is treated with antigen-specific peptide.
背景

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.

Xanthopoulos K.G. Biochem. Biophys. Res. Commun. 215:106-113(1995)
Schalwijk J. Biol. Chem. Hoppe-Seyler 378:373-379(1997)
SeattleSNPs variation discovery resource Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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