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位置: 首页 > 磷酸化抗体 > Claudin 3 (Phospho-Tyr219) Antibody

Claudin 3 (Phospho-Tyr219) Antibody#11791

Claudin 3 (Phospho-Tyr219) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Claudin 3 (Phospho-Tyr219) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 219 (R-K-D-Y(p)-V) derived from Human Claudin 3.

基因/蛋白名称Claudin 3

修饰Phospho

别名CLD3; CLDN3; HRVP1; CPE-R 2; CPE-receptor 2

数据库入口号Swiss-Prot#: O15551;
NCBI Gene#: 1365;
NCBI Protein#: NP_001297.1.

UniprotO15551

实际分子量28kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from COLO cells treated with EGF using Claudin 3 (Phospho-Tyr219) Antibody #11791.The lane on the right is treated with the antigen-specific peptide.
背景

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands.

Peacock R.E., Genomics 46:443-449(1997).
Katahira J., J. Biol. Chem. 272:26652-26658(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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Email Address

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