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位置: 首页 > 磷酸化抗体 > CtBP1 (Phospho-Ser422) Antibody

CtBP1 (Phospho-Ser422) Antibody#11796

CtBP1 (Phospho-Ser422) Antibody
CtBP1 (Phospho-Ser422) Antibody
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产品总价:
产品详情

产品名称CtBP1 (Phospho-Ser422) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of CtBP1 only when phosphorylated at serine 422.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 422(A-P-S(p)-P-G) derived from Human CtBP1.

基因/蛋白名称CtBP1

修饰Phospho

别名CTBP; C-terminal binding protein 1; EC 1.1.1;

数据库入口号Swiss-Prot#: Q13363;
NCBI Gene#: 1487;
NCBI Protein#: NP_001319.1.

UniprotQ13363

实际分子量48kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from Jurkat cells treated with TNF using CtBP1 (Phospho-Ser422) Antibody #11796.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody #11796 (left)or the same antibody preincubated with blocking peptide (right).
背景

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.

Schaeper U., Proc. Natl. Acad. Sci. U.S.A. 92:10467-10471(1995).
Sewalt R.G.A.B., Mol. Cell. Biol. 19:777-787(1999).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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