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位置: 首页 > 磷酸化抗体 > Cyclin C (Phospho-Ser275) Antibody

Cyclin C (Phospho-Ser275) Antibody#11797

Cyclin C (Phospho-Ser275) Antibody
Cyclin C (Phospho-Ser275) Antibody
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产品总价:
产品详情

产品名称Cyclin C (Phospho-Ser275) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of Cyclin C only when phosphorylated at serine 275.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 275(N-G-S(p)-A-N) derived from Human Cyclin C.

基因/蛋白名称Cyclin C

修饰Phospho

别名CG1C; cyclin C; SRB11;

数据库入口号Swiss-Prot#: P24863;
NCBI Gene#: 892;
NCBI Protein#: NP_005181.2.

UniprotP24863

实际分子量37kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 3T3 cells (Lane 2), A549 cells (Lane 3) and Hela cells (Lane 4), using Cyclin C (Phospho-Ser275) Antibody #11797. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cyclin C (Phospho-Ser275) antibody #11797 (left)or the same antibody preincubated with blocking peptide (right).
背景

The protein encoded by this gene is a member of the cyclin family of proteins. The encoded protein interacts with cyclin-dependent kinase 8 and induces the phophorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The level of mRNAs for this gene peaks in the G1 phase of the cell cycle. Two transcript variants encoding different isoforms have been found for this gene.

Lew D.J., Cell 66:1197-1206(1991).
Li H., Genomics 32:253-259(1996).
Mungall A.J., Nature 425:805-811(2003).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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