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位置: 首页 > 磷酸化抗体 > E2A (Phospho-Thr355) Antibody

E2A (Phospho-Thr355) Antibody#11798

E2A (Phospho-Thr355) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称E2A (Phospho-Thr355) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of E2A only when phosphorylated at threonine 355.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 355 (P-S-T(p)-P-V) derived from Human E2A .

基因/蛋白名称E2A

修饰Phospho

别名E12; Tran; TCF3; TFE2; ITF1

数据库入口号Swiss-Prot#: P15923;
NCBI Gene#: 6929;
NCBI Protein#: NP_003191.1.

UniprotP15923

实际分子量67kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human brain tissue using E2A (Phospho-Thr355) antibody #11798 (left)or the same antibody preincubated with blocking peptide (right).
背景

E2A is a transcription factor that plays major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Heterodimers between E2A and tissue-specific basic helix-loop-helix (bHLH) Dimers bind DNA on E-box motifs: 5'- CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Deletions in E2A have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Two alternatively spliced human isoforms have been described.

Kamps M.P., Cell 60:547-555(1990).
Nourse J., Cell 60:535-545(1990).
Grimwood J., Nature 428:529-535(2004).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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