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位置: 首页 > 磷酸化抗体 > Estrogen Receptor-α (Phospho-Ser102) Antibody

Estrogen Receptor-α (Phospho-Ser102) Antibody#11801

Estrogen Receptor-α (Phospho-Ser102) Antibody
Estrogen Receptor-α (Phospho-Ser102) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Estrogen Receptor-α (Phospho-Ser102) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of Estrogen Receptor-α only when phosphorylated at serine 102.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine102(L-N-S(p)-V-S) derived from Human Estrogen Receptor-α.

基因/蛋白名称Estrogen Receptor-α

修饰Phospho

别名ER; ESR; ESR1; ESTR; NR3A1

数据库入口号Swiss-Prot#: P03372;
NCBI Gene#: 2099;
NCBI Protein#: NP_000116.2.

UniprotP03372

实际分子量53kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HuvEc cells (Lane 2), using Estrogen Receptor-α (Phospho-Ser102) Antibody #11801. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Phospho-Ser102) antibody #11801 (left)or the same antibody preincubated with blocking peptide (right).
背景

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.

Greene G.L., Science 231:1150-1154(1986).
Green S., Nature 320:134-139(1986).
Pink J.J., Nucleic Acids Res. 24:962-969(1996).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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