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位置: 首页 > 磷酸化抗体 > Gab2 (Phospho-Tyr643) Antibody

Gab2 (Phospho-Tyr643) Antibody#11802

Gab2 (Phospho-Tyr643) Antibody
Gab2 (Phospho-Tyr643) Antibody
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产品总价:
产品详情

产品名称Gab2 (Phospho-Tyr643) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of Gab2 only when phosphorylated at tyrosine 643.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 643(V-D-Y(p)-V-Q) derived from Human Gab2 .

基因/蛋白名称Gab2

修饰Phospho

别名pp100; GABJ; KIAA0571;

数据库入口号Swiss-Prot#: Q9UQC2;
NCBI Gene#: 9846;
NCBI Protein#: NP_536739.1.

UniprotQ9UQC2

实际分子量74kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from 3T3 cells (Lane 2), using Gab2 (Phospho-Tyr643) Antibody #11802. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Gab2 (Phospho-Tyr643) antibody #11802 (left)or the same antibody preincubated with blocking peptide (right).
背景

This gene is a member of the GRB2-associated binding protein (GAB) gene family and is similar to the GAB1 gene. These proteins contain pleckstrin homology (PH) domain, and bind SHP2 tyrosine phosphatase and GRB2 adapter protein. They act as adapters for transmitting various signals in response to stimuli through cytokine and growth factor receptors, and T- and B-cell antigen receptors. The protein encoded by this gene is the principal activator of phosphatidylinositol-3 kinase in response to activation of the high affinity IgE receptor. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.

Nishida K., Blood 93:1809-1816(1999).
Nagase T., DNA Res. 5:31-39(1998).
Salomon A.R., Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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