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位置: 首页 > 磷酸化抗体 > PAK5/6 (Phospho-Ser602/Ser560) Antibody

PAK5/6 (Phospho-Ser602/Ser560) Antibody#11812

PAK5/6 (Phospho-Ser602/Ser560) Antibody
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产品总价:
产品详情

产品名称PAK5/6 (Phospho-Ser602/Ser560) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous levels of PAK5/6 only when phosphorylated at serine 602/560.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 602/560(R-K-S(p)-L-V) derived from Human PAK5/6.

基因/蛋白名称PAK5/6

修饰Phospho

别名PAK5; PAK 6; kinase PAK6;

数据库入口号Swiss-Prot#: Q9P286/Q9NQU5;
NCBI Gene#: 57144/56924;
NCBI Protein#: NP_065074.1.

UniprotQ9P286/Q9NQU5

实际分子量75kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HeLa cells (Lane 2), using PAK5/6 (Phospho-Ser602/Ser560) Antibody #11812. The lane on the left is treated with antigen-specific peptide.
背景

This gene encodes a protein that shares a high degree of sequence similarity with p21-activated kinase (PAK) family members. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development.

Yang F., J. Biol. Chem. 276:15345-15353(2001).
Wagner T., Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
Sjoeblom T., Science 314:268-274(2006).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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