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位置: 首页 > 磷酸化抗体 > TOB1 (Phospho-Ser164) Antibody

TOB1 (Phospho-Ser164) Antibody#11816

TOB1 (Phospho-Ser164) Antibody
TOB1 (Phospho-Ser164) Antibody
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产品总价:
产品详情

产品名称TOB1 (Phospho-Ser164) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of TOB1 only when phosphorylated at serine 164.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine 164(A-V-S(p)-P-T) derived from Human TOB1.

基因/蛋白名称TOB1

修饰Phospho

别名TOB; Transducer of erbB-2 1;

数据库入口号Swiss-Prot#: P50616;
NCBI Gene#: 10140;
NCBI Protein#: NP_001230806.1.

UniprotP50616

实际分子量40kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from HeLa cells (Lane 2), A549 cells (Lane 3) and HepG2 cells (Lane 4), using TOB1 (Phospho-Ser164) Antibody #11816. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using TOB1 (Phospho-Ser164) antibody #11816 (left)or the same antibody preincubated with blocking peptide (right).
背景

This gene encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.

Matsuda S., Oncogene 12:705-713(1996).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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