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位置: 首页 > 磷酸化抗体 > PLD1 (Phospho-Thr147) Antibody

PLD1 (Phospho-Thr147) Antibody#11818

PLD1 (Phospho-Thr147) Antibody
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产品总价:
产品详情

产品名称PLD1 (Phospho-Thr147) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of PLD1 only when phosphorylated at threonine 147.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 147(R-H-T(p)-F-R) derived from Human PLD1.

基因/蛋白名称PLD1

修饰Phospho

别名 PLD 1; hPLD1; Choline phosphatase 1;

数据库入口号Swiss-Prot#: Q13393;
NCBI Gene#: 5337;
NCBI Protein#: NP_002653.1.

UniprotQ13393

实际分子量120kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from HepG2 cells using PLD1 (Phospho-Thr147) Antibody #11818.The lane on the right is treated with the antigen-specific peptide.
背景

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis.

Hammond S.M., J. Biol. Chem. 272:3860-3868(1997) [PubMed: 9013646].
Steed P.M., FASEB J. 12:1309-1317(1998) [PubMed: 9761774].
Lopez I., J. Biol. Chem. 273:12846-12852(1998) [PubMed: 9582313].

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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