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位置: 首页 > 磷酸化抗体 > FADD (Phospho-Ser191) Antibody

FADD (Phospho-Ser191) Antibody#11820

FADD (Phospho-Ser191) Antibody
FADD (Phospho-Ser191) Antibody
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产品总价:
产品详情

产品名称FADD (Phospho-Ser191) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms

特异性The antibody detects endogenous levels of FADD only when phosphorylated at serine 191.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of Serine191(N-R-S(p)-G-A) derived from Mouse FADD.

基因/蛋白名称FADD

修饰Phospho

别名MORT1 ; FAS-associating death domain-containing protein; Mediator of receptor induced toxicity;

数据库入口号Swiss-Prot#: Q13158;
NCBI Gene#: 14082;
NCBI Protein#: NP_003815.1.

UniprotQ13158

实际分子量25kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using FADD (Phospho-Ser191) antibody #11820 (left)or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from Jurkat cells treated with PMA using FADD (Phospho-Ser191) Antibody #11820.The lane on the right is treated with the antigen-specific peptide.
背景

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Sugano S., Nat. Genet. 36:40-45(2004).
Farmer A., Submitted (MAY-2003).
Venter J.C., Submitted (JUL-2005).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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