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位置: 首页 > 磷酸化抗体 > MAPK1/3 (Phospho-Tyr205/222) Antibody

MAPK1/3 (Phospho-Tyr205/222) Antibody#11825

MAPK1/3 (Phospho-Tyr205/222) Antibody
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产品总价:
产品详情

产品名称MAPK1/3 (Phospho-Tyr205/222) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of MAPK1/3 only when phosphorylated at tyrosine 205/222.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 205/222(K-G-Y(p)-T-K) derived from Human MAPK1/3 .

基因/蛋白名称MAPK1/3

修饰Phospho

别名ERK; ERT1; MAPK 1; MAPK2; ERK2

数据库入口号Swiss-Prot#: P28482/P27361;
NCBI Gene#: 5594/5595;
NCBI Protein#: NP_002736.3.

UniprotP28482/P27361

实际分子量44kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from JK cells using MAPK1/3 (Phospho-Tyr205/222) Antibody #11825.The lane on the right is treated with the antigen-specific peptide.
背景

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

Owaki H., Biochem. Biophys. Res. Commun. 182:1416-1422(1992).
Dunham I., Nature 402:489-495(1999).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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