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位置: 首页 > 磷酸化抗体 > VAV3 (Phospho-Tyr173) Antibody

VAV3 (Phospho-Tyr173) Antibody#11830

VAV3 (Phospho-Tyr173) Antibody
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产品总价:
产品详情

产品名称VAV3 (Phospho-Tyr173) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of VAV3 only when phosphorylated at tyrosine 173.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 173 (E-V-Y(p)-E-D) derived from Human VAV3.

基因/蛋白名称VAV3

修饰Phospho

别名VAV3; FLJ40431; Guanine nucleotide exchange factor VAV3;

数据库入口号Swiss-Prot#: Q9UKW4;
NCBI Gene#: 10451;
NCBI Protein#: NP_006104.4.

UniprotQ9UKW4

实际分子量100kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C/1 year

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from JK cells using VAV3 (Phospho-Tyr173) Antibody #11830.The lane on the right is treated with the antigen-specific peptide.
背景

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Trenkle T., Nucleic Acids Res. 26:3883-3891(1998).
Movilla N., Mol. Cell. Biol. 19:7870-7885(1999).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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