产品详情
产品名称mTOR(Ab-2448) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of total mTOR protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa. 2446~2450 (T-D-S-Y-S) derived from Human mTOR.
基因/蛋白名称mTOR
别名FRAP; FRAP1; FRAP2; RAFT1;
数据库入口号Swiss-Prot: P42345
NCBI Protein: NP_004949.1
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 289kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela, 293 and JK cells using mTOR(Ab-2448) Antibody #21214.
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using mTOR(Ab-2448) Antibody #21214(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed MCF7 cells using mTOR(Ab-2448) Antibody #21214.
背景
Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Albanell J,et al.(2007)Clin Transl Oncol.9(8):484-93.
Huang JJ,et al.
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et al,IvabradIne improved left ventricular function and pressure overload-induced cardiomyocyte apoptosis in a transverse aortic constriction mouse model. In Mol Cell Biochem on 2019 Jan by Yu Y, Hu Z, et al..PMID:29790114,
, (2019),
PMID: 29790114
et al,Effect of electrical stimulation combIned with diet therapy on InsulIn resistance via mTOR signalIng. In Mol Med Rep on 2019 Dec by Huang S, Tang N, et al..PMID:31702811,
, (2019),
PMID: 31702811
et al,5-HT 2 receptor mediates high-fat diet-induced hepatic steatosis and very low density lipoprotein overproduction in rats.In Obes Res Clin Pract. On 2018 Jan - Feb by Li X, Guo K et al..PMID: 27133527,
, (2018),
PMID: 27133527
et al,Inhibition of excessive autophagy and mitophagy mediates neuroprotective effects of URB597 against chronic cerebral hypoperfusion.In Cell Death Dis. On 2018 Jun 28 by Su SH, Wu YF et al..PMID:29955058,
, (2018),
PMID: 29955058
et al,Tunneling Nanotubes Promote Intercellular Mitochondria Transfer Followed by Increased Invasiveness in Bladder Cancer Cells.In Oncotarget on 2017 Feb 28 by Jinjin Lu, Xiufen Zheng,et al..PMID: 28107184,
, (2017),
PMID: 28107184
et al,Propranolol Sensitizes Thyroid Cancer Cells to Cytotoxic Effect of Vemurafenib.In Oncol Rep on 2016 Sep by Wei-Jun Wei , Chen-Tian Shen et al..PMID:27432558,
, (2016),
PMID: 27432558
et al,Long-term Stress with Hyperglucocorticoidemia-induced Hepatic Steatosis with VLDL Overproduction Is Dependent on both 5-HT2 Receptor and 5-HT Synthesis in Liver.In Int J Biol Sci on 2016 Jan 1 by Jihua Fu , Shaoxin Ma et al..PMID: 26884719,
, (2016),
PMID: 26884719
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et al,Effective treatment with combination of peripheral 5鈥恏ydroxytryptamine synthetic inhibitor and 5鈥恏ydroxytryptamine 2 receptor antagonist on glucocorticoid鈥恑nduced whole鈥恇ody insulin resistance with hyperglycemia.In J Diabetes Investig on 2016 Nov by Shaoxin Ma , Tao Li et al..PMID:27177506,
, (2016),
PMID: 27177506
et al,Mechanistic Target of Rapamycin Small Interfering RNA and Rapamycin Synergistically Inhibit Tumour Growth in a Mouse Xenograft Model of Human Oesophageal Carcinoma. In J Int Med Res on 2012 by M M Sun, M Z Zhang, et al..PMID: 23206445,
, (2012),
PMID: 23206445
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