产品详情
产品名称Histone H2A.X(Ab-139) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
应用WB IHC IF
种属反应性Hu
特异性The antibody detects endogenous level of total Histone H2A.X protein.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around aa.137~141 (Q-A-S-Q-E) derived from Human Histone H2A.X.
基因/蛋白名称Histone H2A.X
别名H2A.X; H2AFX; H2a/x; HIST5-2AX;
数据库入口号Swiss-Prot: P16104
NCBI Protein: NP_002096.1
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 15kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
背景
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation
Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6
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et al,PTEN expression in U251 glioma cells enhances their sensitivity to ionizing radiation by suppressing DNA repair capacity. In Eur Rev Med Pharmacol Sci on 2019 Dec by Li HL, Wang CY, et al..PMID:31841199,
, (2019),
PMID: 31841199
et al,Extracts of Zuo Jin Wan, a Traditional Chinese Medicine, Phenocopies 5-HTR1D Antagonist in Attenuating Wnt/尾-catenin Signaling in Colorectal Cancer Cells.In BMC Complement Altern Med on 2017 Nov 28 by Jielu Pan, Yangxian Xu,et al..PMID: 29183322,
, (2017),
PMID: 29183322
et al,N-Myc Downstream-Regulated Gene 2 (Ndrg2) Is Involved in Ischemia-Hypoxia-Induced Astrocyte Apoptosis: A Novel Target for Stroke Therapy.In Mol Neurobiol on 2017 Jul by Yu-Long Ma, Li-Xia Zhang,et al..PMID:27154863,
, (2017),
PMID: 27154863
et al,BASI, A Potent Small Molecular Inhibitor, Inhibits Glioblastoma Progression by Targeting microRNA鈥恗ediated 尾鈥怌atenin Signaling.In CNS Neurosci Ther on 2014 Sep by Zhen-Dong Shi , Xiao-Min Qian et al..PMID: 24810017,
, (2014),
PMID: 24810017
Yan Qing, Yanfang Liang, Qingqing Du et al,Apoptosis induced by trimethyltin chloride in human neuroblastoma cells SY5Y is regulated by a balance and cross-talk between NF-娄脢B and MAPKs signaling pathways,
Arch Toxicol, 87(7):1273篓C1285 (2013),
PMID: 23423712
et al,Apoptosis induced by Trimethyltin chloride in human neuroblastoma cells SY5Y is regulated by a balance and cross-talk between NF-魏B and MAPKs signaling pathways. In Arch Toxicol on 2013 Jul by Yan Qing, Yanfang Liang, et al..PMID: 23423712,
, (2013),
PMID: 23423712
Teng Li, Jing Hu, Gong-Hao He et al,Up-regulation of NDRG2 through nuclear factor-kappa B is required for Leydig cell apoptosis in both human and murine infertile testes.,
Biochimica et Biophysica Acta, 1822(2):301篓C313 (2012),
PMID: 22138128
查看更多
et al,Nuclear Translocation of 尾-Catenin Is Essential for Glioma Cell Survival.In J Neuroimmune Pharmacol on 2012 Dec by Zhendong Shi , Xiaomin Qian, et al..PMID:,
, (2012),
PMID: 22415719
et al,Anti-inflammatory mechanism of taurine against ischemic stroke is related to down-regulation of PARP and NF-魏B. In Amino Acids on 2012 May by Ming Sun, Yumei Zhao, et al..PMID: 21409386
,
, (2012),
PMID: 21409386
et al,Up-regulation of NDRG2 through nuclear factor-kappa B is required for Leydig cell apoptosis in both human and murine infertile testes. In Biochim Biophys Acta on 2012 Feb by Teng Li, Jing Hu, et al..PMID:22138128,
, (2012),
PMID: 22138128
Xiao-han Li, Keiji Kikuchi, Yang Zheng et al,Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma.,
Oral Oncology, 47(3):217-223 (2011),
PMID: 21310648
et al,Cytoplasmic expression of p33ING1b is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma. In Histol Histopathol on 2011 May by Xiao-Han Li, Akira Noguchi, et al..PMID: 21432775,
, (2011),
PMID: 21432775
Xiaohan Li, Takeshi Nishida, Akira Noguchi et al,Decreased nuclear expression and increased cytoplasmic expression of ING5 may be linked to tumorigenesis and progression in human head and neck squamous cell carcinoma,
Journal of Cancer Research and Clinical Oncology, 136( 10), 1573-1583 (2010),
PMID: 20182888
Jennifer S. Dickey,Christophe E. Redon,Asako J. Nakamura et al,H2AX: functional roles and potential applications.,
Chromosoma, 118:683篓C692 (2009),
PMID: 19707781
et al,H2AX: Functional Roles and Potential Applications. In Chromosoma on 2009 Dec by Jennifer S Dickey, Christophe E Redon, et al..PMID: 19707781,
, (2009),
PMID: 19707781
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