PKCth(Ab-676) Antibody - SAB | Signalway Antibody

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PKCth(Ab-676) Antibody#21289

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产品名称PKCth(Ab-676) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of total PKCth protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 674~678 (R-L-S-F-A) derived from Human PKCth.

基因/蛋白名称PKCth

别名 nPKC-theta

数据库入口号Swiss-Prot: Q04759
NCBI Protein: NP_006248.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 80kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from PC12 cells using PKCth(Ab-676) Antibody #21289.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Ab-676) Antibody #21289(left) or the same antibody preincubated with blocking peptide(right).

Immunofluorescence staining of methanol-fixed Hela cells using PKCth(Ab-676) Antibody #21289.

背景

This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Kristof Van Kolen, et al. (2006) FEBS J ; 273: 1843 - 1854.
Martin Villalba, et al. (2002) J. Cell Biol ; 157: 253.
Jie Zhang, et al. (2004) J. Biol. Chem ; 279: 22118 - 22123.
Castro AF, et al. (1998) Am J Physiol Cell Physiol; 275: C113 - C119.

如果您使用该产品21289发表了文章，请通知我们，让我们可以引用您的文献。

et al,PKC 胃-Mediated Ca 2+/NF-AT Signalling Pathway May Be Involved in T-cell Immunosuppression in Coal-Burning Arsenic-Poisoned Population.In Environ Toxicol Pharmacol on 2017 Oct by Qibing Zeng , Peng Luo,et al..PMID: 28823652, , (2017),
PMID: 28823652

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应用

WB免疫印迹
IHC免疫组化
IF免疫荧光
ICC免疫细胞化学
FC流式细胞
IP免疫沉淀
E酶联免疫吸附法
DB免疫斑点法
ChIP染色质免疫沉淀
GICA胶体金免疫层析法
NC阴性对照

种属反应性

Hu人
Ms小鼠
Rt大鼠
Dm果蝇
C线虫
Mk猴
Rb兔
B牛
D狗
P猪
Hm仓鼠
ChHm中国仓鼠
Chk鸡

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PKCth(Ab-676) Antibody#21289

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