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位置: 首页 > 抗体 > IkB-e(Ab-22) Antibody

IkB-e(Ab-22) Antibody#21296

IkB-e(Ab-22) Antibody
IkB-e(Ab-22) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称IkB-e(Ab-22) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total IkB-e protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 20~24 (I-E-S-L-R) derived from Human IkB-e.

基因/蛋白名称IkB-e

别名I-kappa-B-epsilon

数据库入口号Swiss-Prot: O00221
NCBI Protein: NP_004547.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 40kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela, 293 and HepG2 cells using IkB-e(Ab-22) Antibody #21296.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-e(Ab-22) Antibody #21296(left) or the same antibody preincubated with blocking peptide(right).
背景
Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.

Shirane M, et al. (1999) J Biol Chem; 274(40): 28169-74
Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663.
Chen, Z.J. et al. (1996) Cell; 84, 853-862.
Brown, K. et al. (1995) Science; 267, 1485-1488.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com