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位置: 首页 > 抗体 > NFkB-p100(Ab-872) Antibody

NFkB-p100(Ab-872) Antibody#21297

NFkB-p100(Ab-872) Antibody
NFkB-p100(Ab-872) Antibody
NFkB-p100(Ab-872) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称NFkB-p100(Ab-872) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of total NFkB-p100 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.870~874 (S-Q-S-V-E) derived from Human NFkB-p100.

基因/蛋白名称NFkB-p100

别名DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lyt10; NFKB2

数据库入口号Swiss-Prot: Q00653
NCBI Protein: NP_001070961.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 120 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NFkB-p100/p52(Ab-872) Antibody #21297(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using NFkB-p100(Ab-872) Antibody #21297.
Western blot analysis of extracts from HT29 cells using NFkB-p100(Ab-872) Antibody #21297 and the same antibody preincubated with blocking peptide.
背景

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

Shin HM, et al. (2006) EMBO J; 25(1): 129-138.
Li Q, et al. (2005) Proc Natl Acad Sci USA; 102(35): 12425-12430.
Chen C, et al. (2000) Mol Cell Biol; 20(8): 2687-2695.
 

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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