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Paxillin(Ab-88) Antibody#21538

Paxillin(Ab-88) Antibody
Paxillin(Ab-88) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Paxillin(Ab-88) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB IF

种属反应性Hu

特异性The antibody detects endogenous level of total Paxillin protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.86~90 (P-V-Y-G-S) derived from Human Paxillin.

基因/蛋白名称Paxillin

别名PAXI; PXN;

数据库入口号Swiss-Prot: P49023
NCBI Protein: NP_001074324.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 68kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:50~1:100
Western blot analysis of extracts from HUVEC cells using Paxillin(Ab-88) Antibody #21538 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed Hela cells using Paxillin(Ab-88) Antibody #21538.
背景
Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).

Burridge, K. et al. (1992) J. Cell Biol. 119, 893-903.
Bellis, S. L. et al. (1995) J. Biol. Chem. 270, 17437-17441
Schaller, M.D. and Parsons, J.T. (1995) Mol. Cell. Biol. 15, 2635-2645.
Turner, C.E. (2000) J. Cell Sci. 113, 4139-4140.

如果您使用该产品21538发表了文章,请通知我们,让我们可以引用您的文献。

et al,Lipid rafts control human melanoma cell migration by regulating focal adhesion disassembly. In Biochim Biophys Acta on 2013 Dec by Ruifei Wang, Jiajia Bi, et al..PMID: 24055995, , (2013),
PMID: 24055995
et al,Lipid raft regulates the initial spreading of melanoma A375 cells by modulating 尾1 integrin clustering. In Int J Biochem Cell Biol on 2013 Aug by Ruifei Wang, Jiajia Bi,et al..PMID: 23665237, , (2013),
PMID: 23665237
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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