免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 抗体 > Collagen IV Antibody

Collagen IV Antibody#33342

Collagen IV Antibody
Collagen IV Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Collagen IV Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB IHC IF

种属反应性Hu Ms

特异性The antibody detects endogenous levels of total Collagen IV protein.

免疫原类型Peptide

免疫原描述Synthesized peptide derived from human Collagen IV.

基因/蛋白名称Collagen IV

别名COL4A1 NC1 domain; COLLAGEN OF BASEMENT MEMBRANE; ALPHA-1 CHAIN ARRESTEN; alpha 1 type IV collagen preproprotein; collagen IV alpha-1 polypeptide

数据库入口号Swiss-Prot: P02462
NCBI Gene ID: 1282

UniprotP02462

实际分子量160kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

IF 1:100-1:500

Western blot analysis of extracts from HeLa cells, using Collagen IV antibody #33342.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Collagen IV antibody #33342.
背景

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

Mary K. Chelberg, Cancer Res., Sep 1989; 49: 4796 - 4802.
Patricia L. Graham, J. Cell Biol., Jun 1997; 137: 1171.
PJ Lein, J. Cell Biol., Apr 1991; 113: 417.
EC Tsilibary, J. Biol. Chem., Dec 1988; 263: 19112 - 19118.

如果您使用该产品33342发表了文章,请通知我们,让我们可以引用您的文献。

et al,Three-Dimensional Culture Promotes Secretion of Extracellular Matrix Structure Fat Flap with Lipoaspirates in Vitro. In Tissue Eng Part A on 2022 Nov by Jing Zhao, Xin Bi,et al..PMID: 36017621, , (2022),
PMID: 36017621
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com