产品详情
产品名称JAB1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IF
种属反应性Hu
特异性The antibody detects endogenous levels of total JAB1 protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from human JAB1.
基因/蛋白名称JAB1
别名SGN3; COP9; SUBUNIT 3;
数据库入口号Swiss-Prot: Q9UNS2
NCBI Gene ID: 8533
UniprotQ9UNS2
实际分子量47kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Immunofluorescence analysis of HeLa cells, treated with Forskolin (40nM, 30mins), using JAB1 antibody #33411.
Western blot analysis of extracts from LOVO cells, using JAB1 antibody #33411.
Western blot analysis of extracts from A549 cells (Lane 2) and HepG2 cells (Lane 3), using JAB1 antiobdy #33411. The lane on the left is treated with systhesized peptide.
背景
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
George Z. Rassidakis, Clin. Cancer Res., Mar 2003; 9: 1121.
Ricardo V. Lloyd, Am. J. Pathol., Mar 2002; 160: 1171.
Shu Xiao, PNAS, Oct 2006; 103: 16448 - 16453.
Alex Kudrin, J. Biol. Chem., Oct 2006; 281: 29641 - 29651.
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