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Caspase 3 Antibody#21420

Caspase 3 Antibody
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产品总价:
产品详情

产品名称Caspase 3 Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of total Caspase 3 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa.29~33 (S-G-I-S-L) derived from Caspase 3.

基因/蛋白名称Caspase 3

别名Apopain

数据库入口号Swiss-Prot: P42574
NCBI Protein: NP_004337.2

UniprotP42574

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 35 17kd
Western blotting: 1:1000

Western blot analysis of extracts from 293, Hela and MDA cells using Caspase 3 Antibody #21420.
背景

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Gervais, F. G et al.Cell 97: 395-406, 1999.
Nicholson, D. W. et al. (1995) Nature 376, 37-43.

如果您使用该产品21420发表了文章,请通知我们,让我们可以引用您的文献。

et al,Ampelopsin induces apoptosis in HepG2 human hepatoma cell line through extrinsic and intrinsic pathways: Involvement of P38 and ERK.In Environ Toxicol Pharmacol on 2015 Nov by Shimei Qi, Xianjuan Kou et al..PMID: 26476886 , , (2015),
PMID: 26476886
et al,BAD overexpression inhibits cell growth and induces apoptosis via mitochondrial-dependent pathway in non-small cell lung cancer.In Cancer Cell Int on 2013 Jun 1 by Jiang L, Luo M, et al..PMID:23725574, , (2013),
PMID: 23725574
et al,Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection.In BMC Infect Dis on 2012 Nov 28 by Shi W, Li X,et al..PMID:23191987, , (2012),
PMID: 23191987
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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