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UBE1L Antibody#33536

UBE1L Antibody
UBE1L Antibody
UBE1L Antibody
是否有货: 有货
产品总价:
产品详情

产品名称UBE1L Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous levels of total UBE1L protein.

免疫原类型Peptide

免疫原描述Synthesized peptide derived from C-terminal of human UBE1L.

基因/蛋白名称UBE1L

别名D8; UBE2; ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1-like; ubiquitin-activating enzyme E1-related protein

数据库入口号Swiss-Prot: P41226
NCBI Gene ID: 7318

UniprotP41226

实际分子量112kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using UBE1L antibody #33536.
Western blot analysis of extracts from HeLa cells, using UBE1L antibody #33536.
Western blot analysis of extracts from JK cells (Lane 2), using UBE1L antiobdy #33536. The lane on the left is treated with synthesized peptide.
背景
Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.

Sutisak Kitareewan, PNAS, Mar 2002; 99: 3806.
Keun Il Kim, Mol. Cell. Biol., Jan 2006; 26: 472 - 479.
Ian Pitha-Rowe, Cancer Res., Nov 2004; 64: 8109 - 8115.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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