产品详情
产品名称Vimentin Antibody
来源种属Rabbit
克隆性Polyclonal
纯化The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
应用WB IHC
种属反应性Hu Ms Rt
特异性The antibody detects endogenous levels of total vimentin protein.
免疫原类型Peptide
免疫原描述Synthesized peptide derived from Internal of human vimentin.
基因/蛋白名称Vimentin
别名VIME; vimentin;
数据库入口号Swiss-Prot: P08670
NCBI Gene ID: 7431
UniprotP08670
实际分子量53kd
浓度1.0mg/ml
配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Vimentin antibody #33541.
Western blot analysis of extracts from HepG2 cells, treated with Adriamycin (0.5uM, 5hours), using Vimentin antibody #33541.
Western blot analysis of extracts from 293 cells (Lane 2), using Vimentin antiobdy #33541. The lane on the left is treated with synthesized peptide.
背景
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
WB Isaacs, J. Biol. Chem., Oct 1989; 264: 17953 - 17960.
Patric Turowski, Mol. Biol. Cell, Jun 1999; 10: 1997.
Melanie L. Styers, J. Cell Sci., Sep 2006; 119: 3643 - 3654.
Qing-Fen Li, J. Biol. Chem., Nov 2006; 281: 34716 - 34724.
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et al,FOXQ1 promotes pancreatic cancer cell proliferation, tumor stemness, invasion and metastasis through regulation of LDHA-mediated aerobic glycolysis InCell Death DisOn2023 Oct 24byChanghao Wu , Chenglong Zheng et al..PMID:37875474,
, (2023),
PMID: 37875474
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