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位置: 首页 > 磷酸化抗体 > eIF2b(phospho-Ser2) Antibody

eIF2b(phospho-Ser2) Antibody#11512

eIF2b(phospho-Ser2) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称eIF2b(phospho-Ser2) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of eIF2b only when phosphorylated at serine 2.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 2 (M-S(p)-G-D-E) derived from Human eIF2β.

基因/蛋白名称eIF2b

修饰Phospho

别名EIF2S2; EIF2; EIF2B

数据库入口号Swiss-Prot: P20042
NCBI Protein: NP_003899.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 38kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from C2C12 cells untreated or treated with PMA using eIF2b(phospho-Ser2) Antibody #11512.
背景

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Structure of the beta subunit of translational initiation factor eIF-2. The DNA sequence and comparative analysis of human chromosome 20. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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400-9961-933
025-58868422

Email Address

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