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位置: 首页 > 抗体 > Chk2(Ab-516) Antibody

Chk2(Ab-516) Antibody#21117

Chk2(Ab-516) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Chk2(Ab-516) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous levels of total Chk2 protein.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around aa. 514~518(Q-P-S-T-S) derived from Human Chk2.

基因/蛋白名称Chk2

别名CHEK2; RAD53;

数据库入口号Swiss-Prot: O96017
NCBI Protein: NP_001005735.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 62kd
Western blotting: 1:500~1:1000

Western blot analysis of extract from HL60 cells using Chk2(Ab-516) Antibody #21117.
背景

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

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Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com