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位置: 首页 > 磷酸化抗体 > SP1(Phospho-Thr739) Antibody

SP1(Phospho-Thr739) Antibody#11241

SP1(Phospho-Thr739) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称SP1(Phospho-Thr739) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous levels of SP1 only when phosphorylated at threonine 739.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 739 (T-A-T(p)-P-S) derived from human SP1

基因/蛋白名称SP1

修饰Phospho

别名TSFP1; Sp1 transcription factor;

数据库入口号Swiss-Prot: P08047
NCBI Protein: NP_612482.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 90kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from 3T3 cells treated with starvation using SP1(Phospho-Thr739) Antibody #11241.
背景
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-a G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Thiesen HJ,et al.(1990)Nucleic Acids Res.18(11):3203-9.
Zutter MM,et al.(1997) Blood.90(2):678-89.
Lou Z,et al.(2005)Cancer Res.65(3):1007-17

如果您使用该产品11241发表了文章,请通知我们,让我们可以引用您的文献。

et al,Synergistic effects of c-Jun and SP1 in the promotion of TGF尾1-mediated diabetic nephropathy progression.In Exp Mol Pathol on 2016 Jun by Pan Gao, Yingze Wei et al..PMID:27112839, , (2016),
PMID: 27112839
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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