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位置: 首页 > 磷酸化抗体 > CK2a(phospho-Thr360/Ser362) Antibody

CK2a(phospho-Thr360/Ser362) Antibody#11572

CK2a(phospho-Thr360/Ser362) Antibody
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产品总价:
产品详情

产品名称CK2a(phospho-Thr360/Ser362) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of CK2 only when phosphorylated at thronine 360.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine360/serine 362 (V-P-T(p)-P-S(p)-P-L) derived from Human CK2a.

基因/蛋白名称CK2a

修饰Phospho

别名CKII; CK2A1; CSNK2A1

数据库入口号Swiss-Prot: P68400
NCBI Protein: NP_001894.2

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 42kd
Western blotting: 1:1000

Western blot of CK2a(Phospho- Thr360/Ser362) antibody(#11572) and CK2a antibody(#21572) in vitro kinase assay. Both purified ERK2 and CK2 were used. CK2a(Phospho-Thr360/Ser362) antibody could recognize ERK2 phosphorylated wild type CK2a and CK2a when Ser362 was mutated to alanine .
背景

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The a and a' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

Keller D.M., Zeng X., et al., Mol. Cell 7:283-292(2001)
Keller D.M., Lu H. et al., J. Biol. Chem. 277:50206-50213(2002)
Trembley J.H., Tatsumi S., et al., Mol. Cell. Biol. 25:1446-1457(2005)
Niefind K., Guerra B., et al., Acta Crystallogr. D 56:1680-1684(2000)

如果您使用该产品11572发表了文章,请通知我们,让我们可以引用您的文献。

et al,Phenelzine, a small organic compound mimicking the functions of cell adhesion molecule L1, promotes functional recovery after mouse spinal cord injury.In Restor Neurol Neurosci. On 2018 July 22 by Li R, Sahu S et al..PMID: 29889084 , , (2018),
PMID: 29889084
et al,Trimebutine, a small molecule mimetic agonist of adhesion molecule L1, contributes to functional recovery after spinal cord injury in mice. In Dis Model Mech on 2017 Sep 1 by Junping Xu, Chengliang Hu, et al..PMID: 28714852, , (2017),
PMID: 28714852
et al,The intracellular domain of L1CAM binds to casein kinase 2伪 and is neuroprotective via inhibition of the tumor suppressors PTEN and p53.In J Neurochem on 2015 Jun by Yan Wang , Melitta Schachner et al..PMID: 25727698, , (2015),
PMID: 25727698
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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